标题:
长春花细胞生物碱合成培养过程的营养消耗研究The Study of Nutrient Consumption on Catharanthus Roseus Cell in the Alkaloid Biosynthesis Culture
作者:
赵楠, 陈文, 郭志刚
关键字:
长春花, 细胞培养, 生物反应器, 萜类吲哚生物碱, 长春碱Catharanthus Roseus; Cell Culture; Bio-Fermenter; Terpene Indole Alkanoid; Vinblastine
期刊名称:
《Bioprocess》, Vol.2 No.2, 2012-06-25
摘要:
本文利用EPC-500PE型植物细胞反应器培养了长春花细胞，筛选到较优的参数组合为150 r.p.m搅拌速度和0.5 mL/min供气量。在合成培养过程中，长春花细胞的蔗糖消耗量约为初始添加浓度(30 g/L)的50%，硝态氮的消耗量为初始添加浓度的90%，氨态氮的消耗量为初始添加浓度的50%，磷的消耗量为初始添加浓度的70%。长春碱含量在第5天达到峰值。
Catharanthus roseus cells were cultured using the EPC-500 PE bio-fermenter, and a stirring speed of 150 r/min and gas supply of 0.5 ml/min were determined to be the best cultivating conditions. During the cultivation process, the cells’ sucrose consumption was about 50% of the initial addition (30 g/L), while nitrate consumption, ammonia nitrogen consumption and phosphorus consumption were 90%, 50% and 70% of the initial addition, respectively. The content of vinblastine peaked at the fifth day.