Hwang, W.Y., Fu, Y., Reyon, D., Maeder, M.L., Tsai, S.Q., Sander, J.D., et al. (2013) Efficient genome editing in zebrafish using a CRISPR-Cas system. Nature Biotechnology, 31, 227-229.
被以下文章引用:
-
标题:
基因组定向编辑技术——CRISPR/Cas9的研究进展Advances in Genome Directional Editing Technologies of CRISPR/Cas9
作者:
姚瑶, 钱雪艳, 郭东全
关键字:
CRISPR/Cas系统, Cas9蛋白, 基因定点修饰CRISPR/Cas System, Cas9 Protein, Targeted Genome
Modification
期刊名称:
《Hans Journal of Agricultural Sciences》, Vol.4 No.6, 2014-12-15
摘要:
CRISPR/Cas系统(Clustered regularly interspaced short palindromic repeats/CRISPR-associated)是广泛存在于细菌及古生菌中的，由细菌体长期进化而形成的，能够降解入侵病毒或噬菌体DNA的适应性免疫系统。由于CRISPR/Cas的Ⅱ型系统比较简单，因此成为目前应用最为广泛的方法。本文主要介绍了CRISPR/Cas系统的基本结构、作用原理、技术特点、研究进展以及该技术可能的应用前景等方面内容。CRISPR/Cas (Clustered regularly interspaced short palindromic repeats/CRISPR-associated) refers to an adaptive immune system that is gained from the long-term evolution of the organism which is widespread in bacteria and archaea. The system is able to degrade the invading virus or phage DNA. TypeⅡ of CRISPR/Cas system has become the most popular method due to its simplicity. This paper summarizes the basic structure, principle, technical characteristics and the progress of CRISPR/Cas, as well as the application prospect of the technology.