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Jinek, M., East, A., Cheng, A., Lin, S., Ma, E. and Doudna, J. (2013) RNA-programmed genome editing in human cells. Elife, 2, Article ID: e00471.

被以下文章引用:

• 标题: 基因组定向编辑技术——CRISPR/Cas9的研究进展Advances in Genome Directional Editing Technologies of CRISPR/Cas9

  作者: 姚瑶, 钱雪艳, 郭东全

  关键字: CRISPR/Cas系统, Cas9蛋白, 基因定点修饰CRISPR/Cas System, Cas9 Protein, Targeted Genome Modification

  期刊名称: 《Hans Journal of Agricultural Sciences》, Vol.4 No.6, 2014-12-15

  摘要: CRISPR/Cas系统(Clustered regularly interspaced short palindromic repeats/CRISPR-associated)是广泛存在于细菌及古生菌中的，由细菌体长期进化而形成的，能够降解入侵病毒或噬菌体DNA的适应性免疫系统。由于CRISPR/Cas的Ⅱ型系统比较简单，因此成为目前应用最为广泛的方法。本文主要介绍了CRISPR/Cas系统的基本结构、作用原理、技术特点、研究进展以及该技术可能的应用前景等方面内容。CRISPR/Cas (Clustered regularly interspaced short palindromic repeats/CRISPR-associated) refers to an adaptive immune system that is gained from the long-term evolution of the organism which is widespread in bacteria and archaea. The system is able to degrade the invading virus or phage DNA. TypeⅡ of CRISPR/Cas system has become the most popular method due to its simplicity. This paper summarizes the basic structure, principle, technical characteristics and the progress of CRISPR/Cas, as well as the application prospect of the technology.