药用真菌杨树菇SUMO基因及抗肿瘤活性初步鉴定
The Molecular Cloning and Antitumor Activity Characterization of SUMO from Medicinal Fungus Agrocybe aegerita
摘要:
目的:克隆杨树菇抗肿瘤相关蛋白基因,初步鉴定其活性。方法:构建cDNA文库,随机测序得到SUMO (Small Ubiquitin-like Modifier)基因,通过构建真核表达质粒,并检测其在HeLa细胞中表达引起的细胞凋亡及衰老的变化,初步鉴定其抗肿瘤活性。结果:得到全长为606 bp的SUMO cDNA序列,编码103个氨基酸,将表达SUMO的真核表达质粒转染入HeLa细胞中,发现可引起肿瘤细胞的凋亡,但对细胞衰老无影响。结论:药用真菌中SUMO等小泛素类蛋白酶系统值得进一步研究,有助于新型抗肿瘤药物前体的开发。
Objective: The antitumor-associated gene of Agrocybe aegerita was cloned to identify the bioactivity. Method: cDNA library was constructed and the gene SUMO was obtained by random sequencing. The eukaryotic ex- pression vector was constructed to detect cell apoptosis and senescence activity. Result: The gene of SUMO was cloned with 606 bp and 103 amino acid residues. The expression in HeLa cells could induce the cell apoptosis instead of se- nescence. Conclusion: SUMO from medicinal fungus worth to be investigated for the discovery of new antitumor medicine.
参考文献
[1]
|
A. T. Borchers, C. L. Keen and M. E. Gershwin. Mushrooms, tumors, and immunity: An update. Experimental Biology and Medicine (Maywood), 2004, 229(5): 393-406.
|
[2]
|
A. T. Borchers, J. S. Stern, R. M. Hackman, C. L. Keen and M. E. Gershwin. Mushrooms, tumors, and immunity. Proceedings of the Society for Experimental Biology and Medicine, 1999, 221(4): 281-293.
|
[3]
|
B. Z. Zaidman, M. Yassin, J. Mahajna and S. P. Wasser. Medici- nal mushroom modulators of molecular targets as cancer thera- peutics. Applied Microbiology and Biotechnology, 2005, 67(4): 453-468.
|
[4]
|
W. Breene. Nutritional and medicinal value of specialty mush- rooms. Journal of Food Protection, 1990, 53(10): 883-894.
|
[5]
|
T. B. Ng. Peptides and proteins from fungi. Peptides, 2004, 25 (6): 1055-1073.
|
[6]
|
H. X. Wang, N. B. Ng and V. E. C. Ool. Lectins from mush- rooms. Mycological Research. 1998, 102(8): 897-906.
|
[7]
|
Y. Liang, Y. C. Chen, H. H. Liu, R. Luan, T. Che, S. Jiang, D. Y. Xie and H. Sun. The tumor rejection effect of protein compo- nents from medicinal fungus. Biomedicine & Preventive Nutri- tion, 2011, 1(4): 245-254.
|
[8]
|
P. H. Ngai, H. X. Wang and T. B. Ng. Purification and charac- terization of a ubiquitin-like peptide with macrophage stimulat- ing, antiproliferative and ribonuclease activities from the mush- room Agrocybe cylindracea. Peptides, 2003, 24(5): 639-645.
|
[9]
|
H. X. Wang, T. B. Ng. Isolation of a novel ubiquitin-like protein from Pleurotus ostreatus mushroom with anti-human immuno- deficiency virus, translation-inhibitory, and ribonuclease activi- ties. Biochemical and Biophysical Research Communications, 2000, 276(2): 587-593.
|
[10]
|
Y. W. Lam, T. B. Ng and H. X. Wang. Antiproliferative and antimitogenic activities in a peptide from puffball mushroom Calvatia caelata. Biochemical and Biophysical Research Com- munications, 2001, 289(3): 744-749.
|