马尾松松针中黄酮类化合物的提取工艺及抑菌活性研究

Study on Extraction Technology and Antibacterial Activity of Flavonoids from Pinus massoniana

摘要: 通过乙醇浸渍法从马尾松针中提取黄酮类化合物，采用正交实验确定了乙醇浓度、料液比、提取时间、提取温度等最佳的提取技术条件。提取后，回收乙醇，用水分散提取物，用石油醚去除叶绿素，采用正丁醇萃取，减压回收正丁醇，得到总黄酮。采用平板菌落计数法测定总黄酮的抑菌活性。结果表明，最佳提取条件为：乙醇浓度75%，材料比1:30 g/mL，提取时间90 min，提取温度90℃；主要和次要因素的影响是：乙醇浓度 > 萃取时间 = 物质/液体比 > 萃取温度。马尾松松针黄酮类化合物对沙门氏菌、大肠杆菌和金黄色葡萄球菌有抑制作用，其中对沙门氏菌的抑制作用最强。

Abstract: Flavonoids were extracted from Pinus massoniana needles by ethanol impregnation, and the opti-mal extraction technical conditions such as ethanol concentration, ratio of material to liquid, ex-traction time, and extraction temperature were determined by orthogonal experiments. After ex-traction, ethanol was recovered, the extract was dispersed with water, chlorophyll was removed with petroleum ether, n-butanol was extracted using n-butanol, and n-butanol was recovered under reduced pressure to obtain total flavonoids. The antibacterial activity of total flavonoids was deter-mined by plate colony counting. The results showed that the optimum extraction conditions were as follows: ethanol concentration 75%, material ratio 1:30 g/mL, extraction time 90 min, extraction temperature 90˚C; the effects of main and secondary factors were as follows: ethanol concentration > extraction time = substance/liquid ratio > extraction temperature. Pinus massoniana flavonoids in-hibited Salmonella, E. coli and S. aureus, with the strongest inhibition against Salmonella.