烟酸诺氟沙星对乌鳢肝微粒酶活性的影响
The Influence of the Different Doses of Niacin Norfloxacin on the Enzyme Activity of Hepatic Microsome in Channa argus
DOI: 10.12677/OJFR.2016.34014, PDF, HTML, XML, 下载: 1,633  浏览: 3,251  科研立项经费支持
作者: 左梦丽, 沈锦玉*：浙江省淡水水产研究所，浙江省鱼类健康与营养重点实验室，浙江 湖州；上海海洋大学水产与生命学院，上海；姚嘉赟：浙江省淡水水产研究所，浙江省鱼类健康与营养重点实验室，浙江 湖州；尹文林：上海海洋大学水产与生命学院，上海
关键词: 乌鳢；烟酸诺氟沙星；肝微粒体；酶活性；Channa argus； Niacin Norfloxacin； Hepatic Microsome； Enzyme Activity
摘要: 目的：研究不同浓度烟酸诺氟沙星对乌鳢肝微粒体细胞色素P450酶系的影响，旨在为水产养殖合理用药提供安全指导。方法：健康乌鳢60尾，体重200 ± 20.0 g，随机分为空白对照组、低剂量(5 mg/kg)、中剂量(20 mg/kg)、高剂量(40 mg/kg)烟酸诺氟沙星浓度组，每组15尾，连续口灌给药5天。采用差速离心法提取肝微粒体，利用底物代谢法联合紫外分光光度计测定NADPH-细胞色素C还原酶(NCCR)、苯胺-4-羟化酶(AH)、氨基比林-N-去甲基酶(AND)、红霉素-N-脱甲基酶(ERND) 4种酶的含量。结果试验结果表明，与对照组相比，烟酸诺氟沙星组微粒体蛋白的含量明显减少(p < 0.05)，而NCCR、AH、AND、ERND4种酶的活性则显著提高(p < 0.05)。且随着药物浓度的升高，表现出活性增强的趋势。结论：烟酸诺氟沙星对乌鳢肝微粒体细胞色素P450酶系活性具有诱导作用。
Abstract: The present study was conducted to evaluate the effects of niacin norfloxacin on the activity of CYP450 enzyme in liver of Channa argus. The aim of the study was to provide security guidance for rational use of niacin norfloxacin in aquaculture. C. arfus were fed with different concentrations (0 mg/ml, 5 mg/kg, 20 mg/kg, 40 mg/kg) of niacin norfloxacin by oral administration for 5 days. The activities of enzyme of NCCR, AH, AND and ERND were determined. Our results demonstrated that microsomal protein content in niacin norfloxacin groups were decreased significantly as compared with control group, and the activities of NCCR, AH, AND and ERND were significantly increased in a dose-depended model which indicated that niacin norfloxacin could promote the activity of those enzyme.
文章引用：左梦丽, 姚嘉赟, 沈锦玉, 尹文林. 烟酸诺氟沙星对乌鳢肝微粒酶活性的影响[J]. 水产研究, 2016, 3(4): 88-96. http://dx.doi.org/10.12677/OJFR.2016.34014

参考文献

[1] Nebert, D.W. and Russell, D. (2002) Clinical Importance of the Cytochrome P450. The Lancet, 360, 1155-1160. https://doi.org/10.1016/S0140-6736(02)11203-7
[2] 邹文. 药物代谢性相互作用[J]. 食品与药品, 2007, 9(12A): 46-51.
[3] 杨宝峰. 药理学[M]. 6版. 北京: 人民卫生出版社, 2003: 11.
[4] 付鹏, 李宏, 中药对药物代谢酶的影响[J]. 中国临床医生杂志, 2007, 35(2): 51-58.
[5] 赵润英, 王玮, 赵丽妮. 肉豆蔻挥发油对小鼠肝微粒体细胞色素P450的影响[J]. 中国中药杂志, 2009, 34(4): 447.
[6] Tang, J.C., Zhang, J.N., Wu, Y.T., et al. (2006) Effect of the Water Extract and Ethanol Extract from Traditional Chinese Medicines Angel & Asinens~(Oliv.) Diels, Ligusticum Chuanxiong Hort. and Rheum Palmatum Lon Rat Diver Cytochrome P450 Activity. Phytotherapy Research, 20, 1046-1056. https://doi.org/10.1002/ptr.1974
[7] 许黎君, 居文政, 陈为烤. 灯盏细辛注射液对小鼠肝微粒体细胞色素P450含量的影响[J]. 中国临床药理学与治疗学, 2008, 13(10): 1122-1131.
[8] 韦锦斌, 臧林泉, 宁宗. 薤白水提物对小鼠肝微粒体CytP450的影响[J]. 蛇志, 2006, 18(3): 187-195.
[9] 况晓东, 李新华, 熊玉卿. 川芎嗪在大鼠肝微粒体系统中的代谢研究[J]. 中国中药杂志, 2006, 31(23): 1971-1984.
[10] 肖鹏, 周宏灏. 细胞色素氧化酶CYP1A2的研究进展[J]. 中南大学学报(医学版), 2008, 33(5): 456-460.
[11] 周宏灏. 遗传药理学[M]. 北京: 人民军医出版社, 2003: 53-54.
[12] Butler, M.A., Wasaki, M., Guengefich, F.P. and Kadlubar, F. (1989) Human Cytochrome P-450PA (P-450IA2), the Phenacetinodeethylase Is Primarily Responsible for the Hepatic 3 Demethylation of Caffeine and Noxidation of Carcinogenic Arylamines. Proceedings of the National Academy of Sciences of the United States of America, 86, 7696- 7700. https://doi.org/10.1073/pnas.86.20.7696
[13] Eaton, D.L., Gallagher, E.P., Bammler, T.K. and Kunze, K.L. (1995) Role of Cytochrome P4501A2 in Chemical Carcinogenesis: Implications for Human Variability in Expression and Enzyme Activity. Pharmacogenetics, 5, 259-274.
[14] Guengerich, F.P., Parikh, A., Turesky, R.J. and David Josephy, P. (1999) Inter Individual Differences in the Metabolism of Environmental Toxicants: Cytochrome P450 1A2 as a Prototype. Mutation Research, 428, 115-121. https://doi.org/10.1016/S1383-5742(99)00039-3
[15] 廖碧衩. 诺氟沙星在奥尼罗非鱼体内的药代动力学及残留研究[J]. 福建农业学报, 2011, 26(6): 930-934.
[16] 武佰玲, 刘萍. 高月. 天王补心丸中生地黄, 玄参, 天冬和麦冬水提液对大鼠肝CYP450酶含量及其亚型CYP3A, CYP2E 1和CYP1 A2活性的影响[J]. 中国中药杂志, 2011, 36(19): 2710-2714.
[17] Koop, D.R. (1992) Oxidative and Reductive Metabolism by Eytoehrome P4502El. FASEB Journal, 6, 724-730.
[18] 石杰, 王本坚, 杨旭. 体内代谢法研究新药对细胞色素P4501A2和2El的影响[J]. 肝脏, 2008, 13(5): 387-389.
[19] 曲晓蓉, 王印庚, 李胜忠, 等. 诺氟沙星在大菱鲆体内药代动力学及残留消除规律[J]. 海洋水产研究, 2007, 28(5): 25-29.
[20] Hu, X., Li, X.-C., Sun, B.-B., Fang, W.-H., Zhou, S., Hu, L.-L. and Zhou, J.-F. (2011) Effects of Enrofloxacin on Cytochromes P4501A and P4503A in Carassius auratus gibelio (Crucian Carp). Journal of Veterinary Pharmacology and Therapeutics, 35, 216-223. https://doi.org/10.1111/j.1365-2885.2011.01318.x
[21] 戴静. 氟喹诺酮类对猪和鸡肝微粒体CYP1A2活性影响研究[D]: [硕士学位论文]. 武汉: 华中农业大学, 2011.
[22] Mayeaux, M.H. and Winston, G,W. (1998) Antibiotic Ef-fects on Cytochromes P450 Content and Mixed-Function Oxygenase (MFO) Activities in the American Alligator, Al-ligator mississippiensis. Journal of Veterinary Pharmacology and Therapeutics, 21, 274-281. https://doi.org/10.1046/j.1365-2885.1998.00134.x
[23] 陈大健. 鲫鱼肝微粒体细胞色素P450酶系的初步研究[D]: [硕士学位论文]. 南京: 南京农业大学, 2006.
[24] 贾婻. 恩诺沙星在异育银鲫肝微粒体中代谢及对P450主要亚酶影响的初步研究[D]: [硕士学位论文]. 成都: 四川农业大学, 2009.
[25] Silverlight, J., Coldham, N., Thorne, L., Thorne, L. and Jackman, R. (2003) Antibodies to the Quinolones and Fluoroquinolones for the Development of Generic and Specific Immunoassays for Detection of These Residues in Animals Products. Food Additives & Contami, 20, 221-228. https://doi.org/10.1080/0265203021000055388
[26] McLellan, R., Drobitch, R., Monshouwer, M. and Renton, K. (1996) Fluoroquinolone Antibiotics Inhibit Cytochrome P450-Mediated Microsomal Drug Metabolism in Rat and Human. Drug Metabolism and Disposition, 24, 1134-1138.
[27] 王睿, 朱曼, 张永青, 等. 加替沙星和环丙沙星对大鼠肝细胞色素P450酶系的影响[J]. 中国临床药理学杂志, 2004, 20(6): 433-436.
[28] 莫岚, 钱元怒, 王其南, 等. 氟喹诺酮类对大鼠肝微粒体细胞色素P450酶系的影响[J]. 重庆医科大学学报, 1994, 19(4): 274-276.
[29] 胡红芳. 恩诺沙星对鸡细胞色素P450酶系的影响[D]: [博士学位论文]. 上海: 中国人名解放军军事医学科科学院, 2009.
[30] 刘萍, 黄颖, 胡本容, 等. 甲基莲心碱对大鼠肝CYP450酶含量及CYP2D1, CYP3A1, CYP2E1 mRNA的影响[J]. 中国实验方剂学杂志, 2010, 16(10): 161-164.
[31] 姜敏. 甲基莲心碱在大鼠肝微粒体内的代谢与CYP450关系的研究[D]: [硕士学位论文]. 南昌: 江西医学院, 2003.
[32] 黄红兵, 刘韬, 邓多, 等. 紫杉烷类药物对SD大鼠CYP3A1作用效应的研究[J]. 中国药学杂志, 2009, 44(9): 670- 673.
[33] Zhou, H. (2003) Pharmacogenetics. People’s Military Medical Press, Beijing, 53-54.
[34] 瞿颖, 张永旺. P450酶的研究进展[J]. 中国新技术新产品, 2009(16): 7-8.
[35] 卢红丽. 恩诺沙星对小鼠肝微粒体细胞色素P450酶系的影响[M]. 成都: 四川农业大学, 2013.
[36] 刘萍, 边强. 细胞色素P450酶系对药物生物转化的作用[J]. 国外医药合成药生化药制剂分册, 2002, 21(5): 305- 306.
[37] Fuhr, U. (2000) Induction of Drug Metabolizing Enzymes: Pharmacokinetic and Toxicological Consequences in Humans. Clinical Pharmacokinetics, 38, 493-504. https://doi.org/10.2165/00003088-200038060-00003
[38] 魏春燕, 吴逢波, 徐珽. CYP450与药物相互作用[J]. 中国药业, 2014, 23(6): 17-19.
[39] 胡红芳. 恩诺沙星对鸡细胞色素P450酶系的影响[M]. 上海: 中国人民解放军军事医学科学院, 2009.
[40] 翁小刚, 等. 中草药代谢与细胞色素P450的关系研究进展[J]. 中国实验方剂学杂志, 2009, 15(12): 104-108.

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