植物细胞融合及应用概述
The Summary of Plant Cell Fusion and Its Application
DOI: 10.12677/BR.2013.24017, PDF, HTML, XML,  被引量 下载: 4,187  浏览: 15,281  国家科技经费支持
作者: 刘玉鲲, 刘东玉, 周安佩, 何承忠:西南林业大学西南山地森林资源保育与利用省部共建教育部重点实验室,昆明;西南林业大学林学院,昆明
关键词: 植物细胞融合应用 Plant Cell Fusion; Application
摘要:

植物细胞融合已成为植物改良中的重要技术工具。这项技术使得研究者能部分地或全部地结合不同栽培型、不同种、甚至不同属的植物细胞,培养出新型的遗传组合体,包括对称的四倍体体细胞杂合体、非对称的体细胞杂合体或体细胞胞质杂合体。通过克服传统杂交育种中性的不亲合性、多倍性以及雄性不育或雌性不育等难题,植物细胞融合能大大促进植物基因转移和育种的速度。本文简短地总结了植物细胞融合技术及其应用。

Abstract: Plant cell fusion has become an important tool in plant improvement. Plant cell fusion allows researchers to combine somatic cells (partial or whole) from different cultivars, species or genera to generate novel genetic combinations including symmetric allotetraploid somatic hybrids, asymmetric somatic hybrids or somatic cybrids. Through bypassing problems sometimes associated with conventional sexual crossing, including sexual incompatibility, polyembryony and male or female sterility, the plant cell fusion can greatly facilitate the process of gene transfer or breeding. This short review summarizes the plant cell fusion and its application.

文章引用:刘玉鲲, 刘东玉, 周安佩, 何承忠. 植物细胞融合及应用概述[J]. 植物学研究, 2013, 2(4): 99-105. http://dx.doi.org/10.12677/BR.2013.24017

参考文献

[1] [1] 张献龙, 唐克轩. 植物生物技术[M]. 北京: 科学出版社, 2005: 124.
[2] 李春艳, 李妮亚, 陈少良. 植物原生质体培养与融合的研究进展[J]. 海南师范学院学报(自然科学版), 2005, 18(3): 264- 268.
[3] 孙慧慧, 王力军, 闫晓红等. 植物原生质体融合培养技术及其应用[J]. 河南农业科学, 2010, 7(3): 118-122.
[4] 戴雪梅, 黄天带, 孙爱花等. 植物原生质体融合研究进展及其在育种中的应用[J]. 热带作物学报, 2012, 33(8): 1516-1521
[5] 李志勇. 细胞工程[M]. 北京: 科学出版社, 2003: 1-7.
[6] B. P. Jena. Insights on membrane fusion. Cell Biology Interna- tional, 2000, 24(11): 769-771.
[7] 崔彬彬, 朱维红, 冯大领. 细胞融合技术研究进展[J]. 保定师范专科学校学报, 2007, 2: 48-50.
[8] 李保华. 细胞融合技术研究进展[J]. 安徽农业科学, 2008, 15: 6187-6188.
[9] 聂福, 张美萍, 孙春玉等. 五加科植物细胞工程研究进展[J]. 北方园艺, 2010, 7: 206-209.
[10] 谢华永. 杨树胚性悬浮细胞系建立与原生质体融合研究[D]. 南京: 南京林业大学, 2004.
[11] 张献龙, 唐克轩. 植物生物技术[M]. 北京: 科学出版社, 2005: 132.
[12] 汪勋清, 刘录祥. 聚乙二醇诱导细胞融合最适条件的筛选[J]. 核农学报, 2008, 5: 635-639.
[13] 徐文锦, 刘湘, 宁勇. 植物原生质体融合技术的研究进展[J]. 湖北中医学院学报, 2008, 10(1): 46-47.
[14] 郭学民, 徐兴友, 王同坤等. 植物细胞融合的研究进展[J]. 河北科技师范学院学报, 2005, 19(1): 65-68.
[15] 华小黎, 廖婧, 胡丽玲等. 聚乙二醇诱导细胞融合最适条件的筛选[J]. 中国医院药学杂志, 2012, 5: 206-209.
[16] Q. H. Zhang, J. H. Liu and X. X. Deng. Isolation of microproto- plasts from a partially synchronized suspension culture of Citrus unshiu. Journal of Plant Physiology, 163(11): 1185-1192.
[17] C. Ramos, D. Bonenfant and J. Teissie. Cell hybridization by electrofusion on filters. Analytical Biochemistry, 2002, 302(2): 213-219.
[18] M. R. Davey, P. Anthony, J. B. Power, et al. 2004 Sivb Congress symposium proceedings “thinking outside the cell”: Plant protoplast tech-nology: Status and applications. In Vitro Cellular & Develop-mental Biology: Plant, 2005, 41(3): 202-212.
[19] L. F. De Filippislf, R. Hampp and H. Zieglerh. Membrane per- meability changes and ultrastructural abnormalities during pro- toplast fu-sion. Journal of Plant Physiology, 2000, 156(5-6): 628- 634.
[20] W. Jude, G. Fred. Protoplast fusion for production of tetraploids and triploids: applications for scion and rootstock breeding in citrus. Plant Cell, Tissue and Organ Culture, 2011, 104(11): 343- 357.
[21] Y. M. Li, L. J. Guan, L. R. Lou, et al. Laser-induced tobacco protoplast fusion. Science in China, Se-ries C: Life Science, 1999, 42(2): 122-127.
[22] R. Muralidhar, T. Panda. Fungal protoplast fusion—A revisit. Bioprocess Engi-neering, 2000, 22(5): 429-431.
[23] A. Alexander, E. Richard. Somatic hybridization and applica- tions in plant breeding. Plant Breeding Reviews, 2010 20(1): 167-225.
[24] J. Bravo, D. Ev-ans. Protoplast fusion for crop improvement. Plant Breeding Re-views, 2011, 3(1): 193-218.
[25] J. Janick. Somatic embryo-genesis in woody species. Horticul- tural Reviews, 2011, 10(1): 153-181.
[26] F. Liu, Y. G. Wang, J. M. Xue, et al. Transmission measurement based on stmobservation to detect the penetration depth of low energy heavyions in botanic samples. Radiation Measurements, 2003, 37(1): 9-14.
[27] H. Rehm, G. Reed. Cell fusion. Biotechnology Set, 2008, 2(1): 93-139.
[28] 吴紫云, 华玉伟, 黄华孙. 植物微原生质体的融合[J]. 植物生理学通讯, 2007, 43(6): 1182-1187.
[29] 罗立新. 细胞融合技术与应用[M]. 北京: 化学工业出版社, 2003.
[30] 戴永娟, 和兆荣, 胡靖锋等. 青花菜非对称体细胞杂交研究[J]. 湖南农业科学, 2012, 5: 9-12.
[31] 廉玉姬, 林光哲, 赵小梅. 大白菜、青花菜和叶用芥菜体细胞杂交种形态学与细胞学特性鉴定[J]. 园艺学报, 2011, 11: 2099-2110.
[32] H. Yamagishi, M. Landgren, J. Forsberg, et al. Production of asymmetric hybrids between Arabidopsis thaliana and Brassica napus utilizing an efficient protoplast culture system. Theoreti- cal and Applied Genetics, 2002, 104(6-7): 959-964.
[33] 周玲艳, 杨加伟, 梁红. 植物体细胞杂交技术及其在猕猴桃育种中的应用[J]. 仲恺农业工程学院学报, 2009, 22(3): 59-64.
[34] T. Bastia, N. Carotenuto, B. Basile, et al. Induction of novel organelle DNA variation and transfer of resistance to frost and verticil ium wilt in Solanum tuberosum through somatic hy- bridization with iEBN S. commersoni. Euphitica, 2000, 116(1): 1-10.
[35] F. Gil, E. Reytor, D. M. Perez-Filgueira, et al. Mul-timerization of peptide antigens for production of stable immu-nogens in transgenic plants. Journal of Biotechnology, 2007, 128(3): 512- 518.
[36] 闰静, 张明方, 陈利萍. 体细胞杂交技术在蔬菜育种中的研究与应用[J]. 细胞生物学杂志, 2004, 26(1): 51-55.
[37] P. B. Kirtipb, S. Prakashs, K. Gaikwadk, et al. Chloroplast sub- stitution overcomes leaf chlorosis in a Moricandia arvensis- based cytoplasmic male sterile Brassica juncea. Theoretical and Applied Genetics, 1998, 97(7): 1179-1182.
[38] 李培夫. 体细胞杂交技术在作物育种上的开发利用[J]. 种子科技, 2005, 4: 215-216.
[39] J. H. Liuclarkejh, A. M. Chevream, M. Landgrenm, et al. Char- acterization of sexual progenies of male-sterile somatic cybrids between Bras-sica napus and Brassica tournefortii. Theoretical and Applied Genetics, 1999, 99(3-4): 605-610.
[40] M. Wang, Z. Peng, L. Wang, et al. Different rates of chromo- some elimination in symmetric and asymmetric somatic hy- bridization between Festuca arundinacea and Bupleurum scor- zonerifolium. Rus-sian Journal of Plant Physiology, 2011, 58(1): 133-141.
[41] D. Evans, W. Sharp and C. Flick. Plant regeneration from cell cul-tures. Horticultural Reviews, 2011, 3: 214-314.