壳低聚糖对红腺忍冬主要药效成分及产量性状的影响

Effect of Chitosan Oligosaccharide on the Main Medical Components and Yield Characteristics of Lonicera hypoglauca Miq

摘要: 本试验分别用0 mg∙L⁻¹ (CK)、600 mg∙L⁻¹、800 mg∙L⁻¹、1000 mg∙L⁻¹、1200 mg∙L⁻¹壳低聚糖水溶液叶面喷施五年生红腺忍冬，研究了处理后红腺忍冬花蕾的产量及其构成因子以及花、茎、叶中主要药用成分绿原酸、总黄酮、总皂苷含量变化。结果表明，不同处理下大白期单株花蕾产量比对照提高了21.5~101.4%，其中1000 mg∙L⁻¹处理的单株产量最高；800 mg∙L⁻¹~1200 mg∙L⁻¹壳低聚糖提高叶片和茎绿原酸含量达到极显著水平，1200 mg∙L⁻¹对提高花蕾、叶、茎中的总黄酮含量达到显著水平，而600 mg∙L⁻¹浓度提高茎部绿原酸含量的作用最为显著；红腺忍冬不同器官中绿原酸、总黄酮和总皂苷含量顺序是叶 > 花 > 茎。

Abstract: Different concentrations of oligochitosan solutions (0,600, 800, 1000, 1200 mg∙L⁻¹) were sprayed on Lonicera hypoglauca Miq of five years old to study the effects of oligochitosan on its yields, yield com- ponents, and the changes of main effective medical components such as chlorogenic acid, general flavone and total saponins in its flowers, stems, and leaves after different treatments.The results showed that yields of flower buds after those treatments in the great period increased by 21.5% - 101.4% than those of control groups, and it was highest after the 1000 mg∙L⁻¹ treatment. The chlorogenic acid content of leaves and stems increased significantly after the 800 mg∙L⁻¹ - 1200 mg∙L⁻¹ oligochitosan treatments, and the total flavonoid content of leaves and stems reached a remarkable level after 1200 mg∙L⁻¹ treatment. The 600 mg∙L⁻¹ treat- ment, however, had the greatest impact on increasing the general flavone contents of flowers, leaves and stems.The contents of chlorogenic acid, total saponin and flavones in Lonicera hypoglauca Miq were highest in leaves, secondly in flowers, and last in stems.