大鼠视觉诱发电位记录方法比较研究

Comparison of Different Electrodes for F-VEP Recording in Rats

摘要: 目的：比较不同F-VEP检测电极设置方法的差异，为大鼠F-VEP检测探索更优的电极放置方法。方法：SD大鼠18只，随机均分三组，分别予以植入式颅骨电极、传统皮下式针状电极、改良皮下式针状电极记录。检测指标为F-VEP的N1波、P1波的潜伏期和N1-P1波振幅，对结果进行统计学分析。结果：植入式颅骨电极记录组P1波潜伏期较传统皮下式针状电极记录组提前(P = 0.002)，改良皮下式针状电极记录组P1波潜伏期较传统皮下式针状电极记录组提前(P = 0.025)，而植入式颅骨电极组与改良皮下式针状电极组P1波潜伏期无差异(P > 0.05)；植入式颅骨电极记录组N1-P1振幅较皮下式针状电极组均更大(P = 0.028/P = 0.011)，两组皮下式针状电极组振幅无差异(P > 0.05)；安全性方面仅植入式颅骨电极组出现不良事件，皮下针状电极组无一例出现。结论：植入式颅骨电极其敏感性、重复性较优，传统皮下式针状电极安全性较优，而改良皮下式针状电极保留了安全性的优势，同时增强了敏感性和重复性，是对F-VEP检测的有效改良。

Abstract: Objective: To explore a better electrode setting method for F-VEP detection in rats, the difference of different F-VEP recording methods was compared. Methods: 18 SD-rats were randomly divided into three groups. The skull implanted electrode, the traditional hypodermic needle electrode and the modified hypodermic needle electrode were respectively recorded in each group. The recording results are the latency of N1 wave and P1 wave and the amplitude of N1-P1 wave, and were statistically analyzed by SPSS software. Results: The latency of P1 wave in the skull implanted electrode group was significantly advanced than the traditional subcutaneous needle electrode group (P = 0.002); the latency of P1 wave in modified subcutaneous needle electrode group was significantly advanced than the traditional subcutaneous needle electrode group (P = 0.025); and no significantly difference was found between the skull implanted electrode group and the modified subcutaneous needle electrode (P > 0.05). The amplitude of the N1-P1 wave in the skull implanted electrode group were significantly larger than two subcutaneous needle electrode groups (P = 0.028/P = 0.011); and no difference was showed between the two subcutaneous needle electrode group in amplitude of the N1-P1 wave (P > 0.05). Only the skull implanted electrode group had adverse events, while subcutaneous needle electrode groups were all well. Conclusion: The skull implanted electrode recording method has excellent sensitivity and repeatability, and the traditional subcutaneous needle electrode recording method is well in animal’s safety, but the modified subcutaneous needle electrode recording method retains the security advantage and simultaneously enhances the sensitivity and repeatability. So the modified subcutaneous needle electrode recording method is effective in F-VEP recording in rats.