油茶壳提取物对酪氨酸酶的抑制机理

Inhibitory Mechanism of Camellia oleifera Shell Extract on Tyrosinase

摘要: 目的：探究油茶壳提取物对酪氨酸酶的抑制作用，判断其抑制类型，初步探究其抑制机理，并对其活性成分进行测定。方法：分别以L-酪氨酸与L-多巴(L-DOPA)为底物，测定油茶壳提取物对酪氨酸酶单酚酶活性与二酚酶活性的抑制效果，Lineweaver-Burk双倒数作图法探究其抑制机理，并测定油茶壳提取物中的总多酚和黄酮含量。结果：油茶壳提取物对酪氨酸酶单酚酶和二酚酶的IC50分别为0.0711 mg/mL、0.2199 mg/mL，对酶–底物络合物抑制常数KIS为10.7487 mg/mL。油茶壳提取物中总多酚含量为30.22% ± 1.02%，黄酮含量为13.52% ± 0.24%。结论：油茶壳提取物对酪氨酸酶单酚酶与二酚酶均具有较好抑制效果，总多酚、黄酮可能是油茶壳提取物发挥抑制酪氨酸酶活性作用的主要物质。

Abstract: Objective: To explore the inhibitory effect of Camellia oleifera shell extract on tyrosinase, to determine the type of inhibition, to preliminarily explore the inhibition mechanism, and to determine its active components. Method: Using L-tyrosine and L-DOPA as substrates, respectively, the inhibitory effects of Camellia oleifera shell extract on tyrosinase monophenolase activity and diphenolase activity were determined. The Lineweaver-Burk double-reciprocal plot method was used to explore the inhibition mechanism, and the contents of total polyphenols and flavonoids in Camellia oleifera shell extract were determined. Results: The IC50 of Camellia oleifera shell extract for tyrosinase monophenolase and diphenolase were 0.0711 mg/mL and 0.2199 mg/mL, respectively, and the KIS for enzyme-substrate complex was 10.7487 mg/mL. The total polyphenol content in Camellia oleifera shell extract was 30.22% ± 1.02%, and the flavonoid content was 13.52% ± 0.24%. Conclusion: Camellia oleifera shell extract has a good inhibitory effect on tyrosinase monophenolase and diphenolase. Total polyphenols and flavonoids may be the main substances of Camellia oleifera shell extract that inhibit tyrosinase activity.