摘要:
MnFe2O4纳米粒子在核磁共振成像和肿瘤磁热疗的应用上具有很大潜力。但是其应用于生物医学领域的主要障碍是难以制备形貌均一、分散性好、磁性能优异且生物相容性良好的MnFe2O4纳米粒子。目的：通过F127调控下的共沉淀法制备粒径均匀且磁性能较好的MnFe2O4纳米粒子，且通过普朗尼克F127的修饰提高其分散性并降低MnFe2O4纳米粒子的细胞毒性。方法：以F127为模版，采用共沉淀法制备MnFe2O4磁性纳米粒。利用X射线衍射(XRD)、透射电子显微镜(TEM)、振动样品磁强计(VSM)、傅立叶变换红外光谱(FT-IR)等对样品的成分、微观结构、形貌和粒径以及磁性能进行表征。通过MTT实验对样品的细胞毒性进行评价。结果：采用普朗尼克F127调控下的共沉淀法成功制备大约50 nm的球形MnFe2O4铁磁性纳米粒子，其比饱和磁化强度为44.8 emu/g，对HUVEC细胞无明显毒性。结论：共沉淀过程中，F127能够控制MnFe2O4纳米粒子的形貌、粒径，从而提高其磁性能，另外还可以降低其细胞毒性。该方法在生物医学应用上具有很大的潜力。
Abstract:
MnFe2O4 nanoparticles have great potentials in Magnetic Resonance Imaging and tumor hyper-thermia. However for biomedical applications, the main challenges would be of great difficulty to synthesize MnFe2O4 nanoparticles with homogeneous size and morphology, high magnetization, and good biocompatibility. Pluronic F127 was utilized to regulate the coprecipitation process of MnFe2O4 nanoparticles. Objective: The regulation of Pluronic F127 in the coprecipitation process was expected to facilitate the formation of MnFe2O4 nanoparticles with uniform size and morphology, higher magnetization, and low cytotoxicity. Methods: MnFe2O4 nanoparticles were prepared by chemical coprecipitation method in presence of Pluronic F127. X-ray diffraction (XRD), transmission electron microscopy (TEM), and vibrating sample magnetometer (VSM) were used to characterize the composition, microstructure, morphology, and magnetization of the prepared MnFe2O4 nanoparticles. And MTT assays were conducted to investigate their cytotoxicity. Results: The ferromagnetic MnFe2O4 nanoparticles (F127-NPs) were successfully synthesized in the presence of Pluronic F127 via coprecipitation method. Compared to the nanoparticles prepared without Pluronic F127, F127-NPs exhibited a more uniform size of ca. 50 nm and a sphere-like shape. Moreover, F127-NPs possessed a higher magnetization (44.8 emu/g) and exhibited little inhibition to HUVE cells. Conclusion: Pluronic F127 could regulate the coprecipitation process to form the high quality MnFe2O4 nanoparticles and improve the cytocompatibility of nanoparticles. And this method is of a great potential to be applied in the field of biomedicine.