分散液相微萃取技术在氯胺酮检验中的应用

The Dispersive Liquid-Liquid Microextraction Technology in the Application of Ketamine Inspection

摘要: 本文利用分散液相微萃取技术(DLLME)与气相色谱法(GC)相结合，建立检验氯胺酮的定性定量分析的新方法。将含有40.0 µL二氯甲烷(萃取剂)的0.5 mL异丙醇(分散剂)作为萃取体系，快速注入到1.0 mL的水溶液中。分散均匀后，以6000 r/min离心5 min后得到沉于离心管底部尖端的沉淀相(15 ± 0.5 µL)，取底部沉淀相1.0 µL进行气相色谱分析。方法线性范围1.0~900.0 µg/L (r = 0.9996)，检出限0.085 µg/L，相对标准偏差3.5% (n = 3)，加标平均回收率94.2%。

Abstract: By combining DLLME and GC phase, a new method for qualitative and quantitative analysis of the inspection of ketamine is presented. A mixture of 40.0 µL dichloromethane and 0.5 mL isopropyl alcohol is the extraction system. First, we quickly add the mixture to 1.0 mL pure water. Then, we centrifuge for 5 min at 6000 r/min speed in a microcentrifuge. There are 15 ± 0.5 µL precipitated phase at the bottom of the centrifuge tube. Last, we analyze 1 mL precipitated phase by gas chro-matography. The linear range of the method is 1.0 - 900.0 µg/L (r = 0.9996). The detection limit of ketamine is 0.085 µg/L. The relative standard deviation of the determination is 3.5% (n = 3). The average recovery rate is 94.2% to add ketamine into the samples.