粒毛盘菌胞内黑色素的提取工艺、红外光谱及抗氧化活性
Extraction Technology, Infrared Spectra and Antioxidant Activity of Intracellular Melanin from Lachnum
摘要:
采用碱溶酸沉法、超声波辅助提取法以及酶解法提取粒毛盘菌YM404胞内黑色素(LIM404),并在单因素实验的基础上,运用响应面法优化获得超声波辅助法的最佳提取条件为:NaOH浓度1.5mol·L-1、超声功率82 W、提取温度75℃、提取时间33 min,在此条件下提取的黑色素(LIM404-b)得率最高,为12.50%,较碱溶酸沉法获得的黑色素(LIM404-a)、酶解法获得的黑色素(LIM404-c)得率分别提高了5.04%、23.76%。红外光谱显示,LIM404-b的结构特征与LIM404-a、LIM404-c的基本相似。LIM404-b浓度为500mg·L-1时,与1.4251mmol·mL-1 α-生育酚的总抗氧化能力相当,对·OH、DPPH·、·的清除率分别为64.43%、76.05%、68.43%,表明LIM404-b具有较强的抗氧化活性。
Intracellular melanins from Lachnum YM404 (LIM404) were obtained by the methods of alkali solution and acid isolation precipitation, ultrasonic-assisted extraction, enzyme hydrolysis, respectively. Based on the single factor experiments, Box-Behnken experiment was designed to obtain the optimum ultrasonic-assisted extraction conditions of LIM404: NaOH 1.5 mol·L-1, ultrasonic power 82 W, ultrasonic temperature 75˚C and ultrasonic time 33 min. Under these conditions, the yield of melanin (LIM404-b) was 12.5%, and increased 5.04%, 12.15% than that by alkali solution and acid precipitation, enzyme hydrolysis, respectively. FT-IR results showed that the structural characteristics of LIM404-b were similar to that of LIM404-a, LIM404-c. When the LIM404-b concentration was 500 mg·L-1, its total antioxidant capacity was equivalent to α-tocopherol of 1.4251 mmol·mL-1, and the ·OH, DPPH·and · scavenging rates were 64.43%, 76.05%, 68.43%, respectively, suggesting that the antioxidant activity of LIM404-b was strong.
参考文献
[1]
|
Latocha, M., Chodurek, E., Kurkiewicz, S., et al. (2000) Pyro- lytic GC-MS analysis of melanin from black, gray and yellow strains of Drosophila melanogaster. Journal of Analytical and Applied Pyrolysis, 56, 89-98.
|
[2]
|
Dong, C. and Yao, Y. (2012) Isolation, characterization of mela- nin derived from Ophiocordyceps sinensis, an entomogenous fungus endemic to the Tibetan Plateau. Journal of Bioscience and Bioengineering, 113, 474-479.
|
[3]
|
杨善岩, 李海龙, 王升贵等 (2013) 黑芝麻黑色素萃取条件的响应面优化. 浙江农业科学, 1, 77-80.
|
[4]
|
李军, 张乔会, 蓝晶晶等 (2012) 响应面法对山杏核壳黑色素提取工艺的研究. 食品工业科技, 33, 295-299.
|
[5]
|
Liu, Y. and Simon, J.D. (2003) The effect of preparation pro- cedures on the morphology of melanin from the ink sac of sepia officinalis. Pigment Cell Research, 16, 72-80.
|
[6]
|
董华群, 黄剑波, 董华强等 (2012) 超声波辅助提取禾雀花黑色素. 食品研究与开发, 33, 140-143.
|
[7]
|
向洪平, 葛建芳, 张蓝月 (2010) 超声波辅助萃取功能性天然色素的研究与应用进展. 江苏农业科学, 3, 360-362.
|
[8]
|
宋茹, 祝颖, 俞群娣(2009) 酶解法制备的鱿鱼墨黑色素鉴定及理化性质. 浙江海洋学院学报(自然科学版), 28, 95-98.
|
[9]
|
王维维, 袁维风, 薛宏宇等 (2012) 超临界CO2萃取黑色素条件的优化和筛选. 光谱实验室, 29, 2327-2329.
|
[10]
|
Ye, M., Wang, Y., Qian, M.S., et al. (2011) Preparation and properties of the melanin from Lachnum singerianum. Inter- national Journal of Basic and Applied Sciences, 11, 51-58.
|
[11]
|
Ye, M., Chen, X., Li, G.W., et al. (2011) Structural charac- teristics of pheomelanin-like pigment from lachnum singeri- anum. Advanced Materials Research, 284-286, 1742-1745.
|
[12]
|
Ye, M., Wang, Y., Guo, G.Y., et al. (2012) Physicochemical characteristics and antioxidant activity of arginine-modified me- lanin from Lachnum YM-346. Food Chemistry, 135, 2490-2497.
|
[13]
|
Wang, H.Y., Jiang, X.L. and Mu, H.J. (2007) Structure and pro- tective effect of exopolysaccharide from P. Agglomerans strain KFS-9 against UV radiation. Microbiological Research, 162, 124-129.
|
[14]
|
Turkoglu, A., Duru, M.E., Nazime, M., et al. (2007) Antioxidant and antimicrobial activities of Laetiporus sulphureus (Bull.) Murrill. Food Chemistry, 101, 267-273.
|
[15]
|
Xu, Y. and Guo, Z.J. (2008) Study on antioxidant activity of extracts from aconitum taipeicu. Research and Practice of Chinese Medicines, 22, 38-40.
|
[16]
|
Vilkhu, K., Mawson, R., Simons, L., et al. (2008) Applications and opportunities for ultrasound assisted extraction in the food industry—A review. Innovative Food Science & Emerging Tech- nologies, 9, 161-169.
|
[17]
|
Patist, A. and Bates, D. (2008) Ultrasonic innovations in the food industry: From the laboratory to commercial production. Innovative Food Science & Emerging Technologies, 9, 147-154.
|
[18]
|
Tiwari, B.K., O’Donnell, C.P. and Cullen, P.J. (2009) Effect of sonication on retention of anthocyanins in blackberry juice. Journal of Food Engineering, 93, 166-171.
|
[19]
|
徐磊, 钱振明, 孙继波等 (2009) 短梗霉黑色素的分离纯化及结构的初步分析. 氨基酸和生物资源, 31, 9-13.
|
[20]
|
Olennikov, D.N., Agafonova, S.V., Stolbikova, A.V., et al. (2011) Melanin of Laetiporus sulphureus (Bull. Fr.) Murr sterile form. Applied Biochemistry and Microbiology, 47, 298-303.
|
[21]
|
Wang, J., Zhang, Q.B., Zhang, Z.S., et al. (2008) Antioxidant activity of sulfated polysaccharide fractions extracted from Laminaria japonica. International Journal of Biological Macro- molecules, 42, 127-132.
|
[22]
|
叶明, 陈晓, 朱立等 (2010) 暗盘孢属YM421 黑色素稳定性及其抗氧化活性. 菌物学报, 29, 254-260.
|
[23]
|
Kim, D.O., Lee, K.W., Lee, H.J., et al. (2002) Vitamin C equivalent antioxidant capacity (VCEAC) of phenolic phyto- chemicals. Journal of Agricultural and Food Chemistry, 50, 3713-3717.
|