颅骨修复钛网表面PDA/CPP涂层的制备及性能研究

Study on Preparation and Properties of PDA/CPP Bilayer on Skull Repairing Titanium Mesh

摘要: 本文意在探究一种简易高效的应用于颅骨修复钛网表面的生物化学改性方法。首先通过浸泡法在钛网表面沉积聚多巴胺薄膜层(PDA)，其次利用PDA的超强粘附性和二次修饰功能，进一步接枝酪蛋白磷酸肽(CPP)，制备具有优良亲水性及优良生物矿化能力的复合涂层。利用XPS、AFM、接触角分析仪进行涂层表征；浸泡模拟体液(SBF)，利用FESEM及EDS分析体外仿生矿化效果。结果显示，“两步浸泡法”成功将PDA与CPP依次修饰于颅骨修复钛网，无毒性元素引入；与未修饰表面相比，具有PDA/CPP生物活性涂层的表面亲水性得到显著提高，并且能够在1天之内诱导沉积均匀稳定的类骨羟基磷灰石(HA)。

Abstract: To obtain excellent hydrophilicity and superb biomineralization capability, polydopamine/casein phosphopeptides (PDA/CPP) bilayer was prepared through a simple two-step immersion process. The adhering PDA interlayer linked the natural biomolecules, CPP, to the SLA titanium surface via covalent bonds. The bilayer was characterized by AFM, XPS and contact angle analyzer. FESEM and EDS were utilized for analyzing the effect of biomimetic mineralization. Our results indicated that PDA/CPP was successfully coated on skull repairing titanium mesh through two-step immersion without any toxic element induced. The bilayer enhanced the hydrophilicity and the rate of bio-mineralization significantly.