拟南芥At2g34610基因编辑和过表达载体的构建
Construction of Arabidopsis At2g34610 Gene Editing and Overexpression Vector
DOI: 10.12677/BR.2022.114057, PDF, 下载: 253  浏览: 386  科研立项经费支持
作者: 赵一君, 李美玉, 王昕宇, 张 菡, 薛 明, 李桂民*：临沂大学生命科学学院，山东 临沂
关键词: 拟南芥；ABA；At2g34610；基因编辑；过表达；Arabidopsis thaliana； ABA； At2g34610； Gene-Editing； Overexpression
摘要: ABA参与的信号转导可调节植物生长发育的各个阶段，如种子萌发、气孔关闭、根的发育和衰老等，并调控植物对干旱、冷、热、盐渍等多种逆境的适应。研究ABA信号转导通路对了解植物生长发育的调节机制具有极其重要的意义。RT-PCR检测结果显示，At2g34610是ABA响应基因，其表达受ABA诱导上调。为了研究At2g34610的功能，我们分别构建了At2g34610过表达载体和多靶点的基因编辑载体，这些载体可为创制目的基因过表达和突变体植株奠定基础，进而可为探究At2g34610在ABA信号转导和调控拟南芥抗逆性中的作用及其分子机制提供理想的实验材料。
Abstract: The signal transduction involved in ABA regulates various stages of plant growth and develop-ment, such as seed germination, stomatal closure, root development and senescence, and regu-lates plant adaptation to drought, cold, heat, salt and other stresses. The study of ABA signal transduction pathway is of great significance for understanding the regulation mechanism of plant growth and development. The results of RT-PCR showed that At2g34610 was an ABA-responsive gene and its expression was up-regulated by ABA. In order to study the function of At2g34610, gene multi-target editing vector and overexpression vector were constructed respectively. The vector construction laid a foundation for the creation of target gene overexpression and mutant plants, which are ideal experimental materials for exploring the role and molecular mechanism of At2g34610 in ABA signal transduction and regulation of stress resistance in Arabidopsis thaliana.
文章引用：赵一君, 李美玉, 王昕宇, 张菡, 薛明, 李桂民. 拟南芥At2g34610基因编辑和过表达载体的构建[J]. 植物学研究, 2022, 11(4): 486-493. https://doi.org/10.12677/BR.2022.114057

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