摘要:
目的:明确GSK-3β在流感病毒感染过程中所发挥的作用。方法:我们应用RNA干扰技术下调GSK-3β的表达水平后,用TCID50法测定A549细胞上清中的流感病毒滴度,并使用CCK-8细胞活力检测试剂盒和Caspase-Glo凋亡检测试剂盒检测了GSK-3β对流感病毒感染A549细胞活力及流感病毒诱导的A549细胞凋亡的影响。结果:下调GSK-3β的表达降低了流感病毒感染A549细胞上清中的病毒滴度,并且GSK-3β的表达下调还降低了流感病毒感染A549细胞的细胞活力同时增强了流感病毒诱导的细胞凋亡。结论:GSK-3β对流感病毒诱导的宿主细胞凋亡具有负调节作用并且是病毒在细胞中高效复制所必需的。
Abstract:
Objective: In order to clarify the role of glycogen synthase kinase-3β (GSK-3β) in the process of influenza virus infection. Methods: We preformed the RNAi to knock down the GSK-3β gene expression, then we determined the virus titers in the A549 cell supernatants by performing TCID 50 assay, and examined the influence of GSK-3β to cell viabilities of the infected cells as well as the influenza virus-induced A549 cell apoptosis using CCK-8 Kit and Caspase-Glo 3/7 Kit. Results: The results demonstrated that down-regulation of GSK3 expression decreased the viral titers in the A549 cells supernatants. Down-regulation of GSK-3β expression decreased the A549 cell viability, at the same time, increased the influenza virus induced cell apoptosis. Conclusion: Hence, GSK-3β negatively regulated the influenza virus-induced host cell apoptosis, and it was essential for the efficient replication of influenza virus.